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A new species of Fizesereneia Takeda & Tamura, 1980 (Crustacea: Brachyura: Cryptochiridae) from the Red Sea and Oman Naturalis
Meij, S.E.T. van der; Berumen, M.L.; Paulay, G..
A new species of cryptochirid crab, Fizesereneia panda van der Meij, is described and illustrated based on specimens collected from the scleractinian corals Lobophyllia cf. hemprichii and L. cf. corymbosa from the Farasan Banks, Farasan Islands, and the reefs off Thuwal in the Saudi Arabian Red Sea, and from Symphyllia recta from reefs in the Gulf of Oman. This is the second cryptochirid species with the Red Sea as type locality. It can be separated from its congeners by the subrectangular carapace, raised midline and the complete division of the carapace depressions, and reddish black colour pattern of these concavities in live specimens. This new species is the seventh assigned to Fizesereneia. A DNA barcode for the new species has been deposited in...
Tipo: Article / Letter to the editor Palavras-chave: DNA barcoding; Gall crabs; Host specificity; Lobophylliidae; Saudi Arabia; Scleractinia; Taxonomy; 42.74.
Ano: 2015 URL: http://www.repository.naturalis.nl/record/533931
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Biodiversity inventory of the grey mullets (Actinopterygii: Mugilidae) of the Indo‐Australian Archipelago through the iterative use of DNA‐based species delimitation and specimen assignment methods ArchiMer
Delrieu‐trottin, Erwan; Durand, Jean‐dominique; Limmon, Gino; Sukmono, Tedjo; Kadarusman,; Sugeha, Hagi Yulia; Chen, Wei‐jen; Busson, Frédéric; Borsa, Philippe; Dahruddin, Hadi; Sauri, Sopian; Fitriana, Yuli; Zein, Mochamad Syamsul Arifin; Hocdé, Régis; Pouyaud, Laurent; Keith, Philippe; Wowor, Daisy; Steinke, Dirk; Hanner, Robert; Hubert, Nicolas.
DNA barcoding opens new perspectives on the way we document biodiversity. Initially proposed to circumvent the limits of morphological characters to assign unknown individuals to known species, DNA barcoding has been used in a wide array of studies where collecting species identity constitutes a crucial step. The assignment of unknowns to knowns assumes that species are already well identified and delineated, making the assignment performed reliable. Here, we used DNA‐based species delimitation and specimen assignment methods iteratively to tackle the inventory of the Indo‐Australian Archipelago grey mullets, a notorious case of taxonomic complexity that requires DNA‐based identification methods considering that traditional morphological identifications...
Tipo: Text Palavras-chave: Coral Triangle; Cryptic diversity; DNA barcoding; Reference library; Taxonomic gap.
Ano: 2020 URL: https://archimer.ifremer.fr/doc/00606/71810/70300.pdf
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Comparative tadpole morphology in three species of frogs of the genus Spinomantis (Amphibia: Mantellidae) Naturalis
Vejarano, S.; Thomas, M.; Vences, M..
We describe the tadpoles of three species of Malagasy frogs, classified in the genus Spinomantis, based on specimens identified by DNA barcoding. The tadpole of Spinomantis aglavei is a typical Orton type IV larva. The oral disc is not emarginated laterally, but has two mid-ventral folds and a labial tooth row formula of 3(2- 3)/2(1). The tadpole of S. phantasticus is similar, the oral disc being laterally emarginated and having one medial fold; LTRF is 3/3(1). A third species, S. cf. fimbriatus is also similar to the other two species; the oral disc is not laterally emarginated but has three medial folds and LTRF is 3(2-3)/3(1). One shared character is a median fold or emargination in the lower part of the oral disc, although the shape of this fold is...
Tipo: Article / Letter to the editor Palavras-chave: Anura; Spinomantis aglavei; Spinomantis phantasticus; Spinomantis fi mbriatus; Madagascar; Systematics; DNA barcoding; 42.82.
Ano: 2006 URL: http://www.repository.naturalis.nl/record/210775
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Current Methods for Seafood Authenticity Testing in Europe: is there a Need for Harmonisation? ArchiMer
Griffiths, Andrew M.; Sotelo, Carmen G.; Mendes, Rogerio; Perez-martin, Ricardo I.; Schroeder, Ute; Shorten, Marc; Silva, Helena A.; Verrez-bagnis, Veronique; Mariani, Stefano.
Mislabelling of food products has recently received a great deal of public scrutiny, but it remains unclear exactly what methods are being utilised in laboratories testing the authenticity of foods. In order to gain insight into the specific area of the analysis of seafood, a questionnaire focusing on the taxonomic groups typically analysed and the techniques utilised was sent to over one hundred accredited laboratories across the UK, Ireland, Spain, Portugal, France and Germany. Forty-five responded positively, demonstrating significant differences in both the species analysed and methods utilised among the countries included in the survey. Indeed, a diversity of methods was employed across laboratories and efforts to harmonise and/or standardise testing...
Tipo: Text Palavras-chave: Food testing; Forensically informative nucleotide sequencing; Species identification; DNA barcoding; Fisheries.
Ano: 2014 URL: https://archimer.ifremer.fr/doc/00188/29918/28383.pdf
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Diversity, external morphology and ‘reverse taxonomy’ in the specialized tadpoles of Malagasy river bank frogs of the subgenus Ochthomantis (genus Mantidactylus) Naturalis
Randrianiaina, R.-D.; Strauß, A.; Glos, J.; Glaw, F.; Vences, M..
We provide detailed morphological descriptions of the tadpoles of Malagasy river bank frogs of the subgenera Ochthomantis and Maitsomantis (genus Mantidactylus, family Mantellidae), and data on relative abundance and habitat preferences of Ochthomantis species from Ranomafana National Park in southeastern Madagascar. Our study includes the tadpoles of six described and four undescribed candidate species. Eight of these larvae were previously unknown. Tadpoles were identified by DNA barcoding. Due to the very rudimentary taxonomic knowledge on Ochthomantis, we followed a ‘reverse taxonomy’ approach in which adult classification was to a great part determined on the basis of larval differences. By this procedure we even identified one candidate species whose...
Tipo: Article / Letter to the editor Palavras-chave: Amphibia; Mantellidae; Madagascar; Tadpole morphology; DNA barcoding; 42.82.
Ano: 2011 URL: http://www.repository.naturalis.nl/record/369710
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DNA barcodes for soil animal taxonomy PAB
Rougerie,Rodolphe; Decaëns,Thibaud; Deharveng,Louis; Porco,David; James,Sam W.; Chang,Chih-Han; Richard,Benoit; Potapov,Mikhail; Suhardjono,Yayuk; Hebert,Paul D.N..
The biodiversity of soil communities remains very poorly known and understood. Soil biological sciences are strongly affected by the taxonomic crisis, and most groups of animals in that biota suffer from a strong taxonomic impediment. The objective of this work was to investigate how DNA barcoding - a novel method using a microgenomic tag for species identification and discrimination - permits better evaluation of the taxonomy of soil biota. A total of 1,152 barcode sequences were analyzed for two major groups of animals, collembolans and earthworms, which presented broad taxonomic and geographic sampling. Besides strongly reflecting the taxonomic impediment for both groups, with a large number of species-level divergent lineages remaining unnamed so far,...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Collembola; Cryptic diversity; DNA barcoding; Oligochaeta; Species identification; Taxonomic crisis; Taxonomic impediment.
Ano: 2009 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-204X2009000800002
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DNA barcoding: a molecular appliance for fish species identification support Sci. Agrar.Paran. / SAP
Tresbach, Rafael Hencke; Cerqueira, Natalia Menezes; Medeiros, Sarah Ramos; Gutierrez, Harold Julián Pérez; Hernández, Natalia Ossa; Rodrigues, Marília Danyelle Nunes.
The identification of genus and species is made by systematic, by noticing morphological and anatomical characteres which were the phenotype expression. However, changes in one or more gene bases can result in phenotypes similarities between different species, inducing an identification error. Alternatively, molecular appliances based in mithocondrial and ribosomal genes appeared. This study aims to compile information about the molecular identification and present the South American Catfish (Rhamdia quelen) case, for which there is controversy generated by taxonomy identification. The different genes used to identification have their particularities, like insertions and deletions on the case of 16S rRNA and the 18S rRNA. Besides these ribosomal genes,...
Tipo: Info:eu-repo/semantics/article Palavras-chave: DNA barcoding; Peixes; Rhamdia quelen; COI; DNA mitocondrial; DNA ribossomal.
Ano: 2015 URL: http://e-revista.unioeste.br/index.php/scientiaagraria/article/view/10408
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DNA barcoding in Mucorales: an inventory of biodiversity Naturalis
Walther, G.; Pawłowska, J.; Alastruey-Izquierdo, A.; Wrzosek, M.; Rodriguez-Tudela, J.L.; Dolatabadi, S.; Chakrabarti, A.; Hoog, G.S. de.
The order Mucorales comprises predominantly fast-growing saprotrophic fungi, some of which are used for the fermentation of foodstuffs but it also includes species known to cause infections in patients with severe immune or metabolic impairments. To inventory biodiversity in Mucorales ITS barcodes of 668 strains in 203 taxa were generated covering more than two thirds of the recognised species. Using the ITS sequences, Molecular Operational Taxonomic Units were defined by a similarity threshold of 99 %. An LSU sequence was generated for each unit as well. Analysis of the LSU sequences revealed that conventional phenotypic classifications of the Mucoraceae are highly artificial. The LSU- and ITS-based trees suggest that characters, such as rhizoids and...
Tipo: Article / Letter to the editor Palavras-chave: Backusella; Biodiversity; Clinical relevance; DNA barcoding; Intraspecific variability; ITS; LSU; Mucor; Mucorales; Nomenclature; Rhizomucor; Taxonomy; Zygorhynchus.
Ano: 2013 URL: http://www.repository.naturalis.nl/record/532353
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DNA barcoding resolves species complexes in Stigmella salicis and S. aurella species groups and shows additional cryptic speciation in S. salicis (Lepidoptera: Nepticulidae) Naturalis
Nieukerken, E.J. van; Mutanen, M.; Doorenweerd, C..
We sequenced the mitochondrial barcoding marker COI and nuclear marker EF1-alpha for most Nordic and other European species of the Stigmella salicis and S. aurella species groups. In the S. salicis group both markers confirm the synonymy of S. lappovimella with S. zelleriella. Specimens previously identified as Stigmella salicis and S. vimineticola are shown to form a complex of several cryptic species for which the taxonomy needs to be worked out. The species previously recorded as S. vimineticola from Norway represents probably an unnamed species. In the S. aurella group, the oligophagous Rosaceae feeders S. aurella and S. poterii are confirmed to be each a single oligophagous species. The synonymy between Stigmella ulmariae from Filipendula ulmaria and...
Tipo: Article / Letter to the editor Palavras-chave: DNA barcoding; Phylogeny; Lepidoptera; 42.75.
Ano: 2012 URL: http://www.repository.naturalis.nl/record/417740
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Evidence for the validity of Protatlanta sculpta (Gastropoda: Pterotracheoidea) Naturalis
Wall-Palmer, D.; Burridge, A.K.; Peijnenburg, K.C.T.A.; Janssen, A.; Goetze, E.; Kirby, R.; Hart, M.B.; Smart, C.W..
The genus Protatlanta is thought to be monotypic and is part of the Atlantidae, a family of shelled heteropods. These microscopic planktonic gastropods are poorly known, although research on their ecology is now increasing in response to concerns about the effects of ocean acidification on calcareous plankton. A correctly implemented taxonomy of the Atlantidae is fundamental to this progressing field of research and it requires much attention, particularly using integrated molecular and morphological techniques. Here we use DNA barcoding, shell morphology and biogeography to show that the genus Protatlanta includes at least two valid species in the Atlantic Ocean. Protatlanta souleyeti and Protatlanta sculpta were found to be separate species, with...
Tipo: Article / Letter to the editor Palavras-chave: Atlantic Ocean; Biogeography; DNA barcoding; Morphometrics; Protatlanta; Shelled heteropod.
Ano: 2016 URL: http://www.repository.naturalis.nl/record/619150
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Evolutionary history of the sequestrate genus Rossbeevera (Boletaceae) reveals a new genus Turmalinea and highlights the utility of ITS minisatellite-like insertions for molecular identification Naturalis
Orihara, T.; Lebel, T.; Ge, Z.-W.; Smith, M.E.; Maekawa, N..
The sequestrate (truffle-like) basidiomycete genera Rossbeevera, Chamonixia, and Octaviania are closely related to the epigeous mushroom genera Leccinum and Leccinellum. In order to elucidate the properties and placement of several undescribed sequestrate taxa in the group and to reveal the evolutionary history of Rossbeevera and its allies, we conducted phylogenetic analyses based on three nuclear (ITS, nLSU, EF-1α) and two mitochondrial DNA loci (ATP6 and mtSSU) as well as precise morphological observations. Phylogenetic analyses of three nuclear loci suggest a complex evolutionary history with sequestrate fruiting bodies present in several clades, including a previously unrecognized sister clade to Rossbeevera. Here we propose a new sequestrate genus,...
Tipo: Article / Letter to the editor Palavras-chave: Biogeography; Cryptic species; DNA barcoding; Hypogeous fungi; Introgression; Species tree.
Ano: 2016 URL: http://www.repository.naturalis.nl/record/611857
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External morphology of immature stages of Zaretis strigosus (Gmelin) and Siderone galanthis catarina Dottax and Pierre comb. nov., with taxonomic notes on Siderone (Lepidoptera: Nymphalidae: Charaxinae) Rev. Bras. entomol.
Dias,Fernando Maia Silva; Oliveira-Neto,José Francisco de; Casagrande,Mirna Martins; Mielke,Olaf Hermann Hendrik.
ABSTRACT The external morphology of immature stages of Zaretis strigosus (Gmelin, [1790]) and Siderone galanthis catarina Dottax and Pierre, 2009 comb. nov. from southern Brazil are described. Additionally, morphology of the adults and sequences of the mitochondrial gene cytochrome oxidase, subunit I, were analyzed in order to evaluate the taxonomy of Siderone galanthis Hübner, [1823]. Immatures were collected on Casearia sylvestris (Salicaceae) in Curitiba, Paraná, and Balneário Barra do Sul, Santa Catarina, Brazil, and reared at the laboratory. Morphological descriptions and illustrations are provided, based on observations through stereoscopic and optic microscopes attached to camera lucida; results are compared and discussed and immature stages of some...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Anaeini; Chaetotaxy; DNA barcoding; Leafwing; Zaretidinae.
Ano: 2015 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0085-56262015000400307
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First record of the sedge feeder Bactra verutana Zeller (Lepidoptera: Tortricidae) in Chile based on morphology and DNA barcodes Rev. Bras. entomol.
Vargas,Héctor A.; Vargas-Ortiz,Marcelo.
Abstract The sedge-feeding moth Bactra verutana Zeller, 1875 (Lepidoptera: Tortricidae: Olethreutinae: Bactrini), described from Dallas, Texas, USA, is widespread, recorded throughout much North America, Central and South America, including the Caribbean, and Africa. The species is recorded for the first time from Chile based on specimens collected in the coastal valleys of the Atacama Desert, where its larvae feed on Cyperus corymbosus Rottb. var. subnodosus (Nees & Meyen) Kük. (Cyperaceae). A single DNA barcode haplotype, which is widespread in USA, was found in two Chilean specimens sequenced.
Tipo: Info:eu-repo/semantics/other Palavras-chave: Atacama Desert; Cyperaceae; Cyperus corymbosus; DNA barcoding.
Ano: 2019 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0085-56262019000200104
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Lack of correlation between micro fungi species and chemical control method of Atta treated with toxic baits Ciência Rural
Barbosa,Marcílio Souza; Barbosa,Suzanne Nunes; Nagamoto,Nilson Satoru; Forti,Luiz Carlos; lmeida,Cícero.
ABSTRACT: Atta sexdens rubropilosa (leaf-cutter ants) has a symbiotic association with a fungus and has a negative interaction with other fungi due to parasitism of the fungus cultivated by ants; also, there are several other fungi with no exact known role occurring in their cultivated fungus garden. In the present study, we use the ITS region (internal transcribed spacer) to identify fungi in colonies treated with toxic baits. Experiments using two toxic baits were carried out: 0.75g of sulfluramid [0.3%] and 0.75g fipronil [0.003%]. Samples of fungi were collected and cultured in Czapek medium for seven days to allow fungal growth and subsequent identification. Total DNA was isolated from 100-150 mg of mycelium using the CTAB method and using PCR, with...
Tipo: Info:eu-repo/semantics/article Palavras-chave: ITS; DNA barcoding; Leaf-cutter; Ants.
Ano: 2018 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782018000500150
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Markets, mixtures and molecular methods: investigating medicinal plant and edible orchid diversity in Tanzania and Zambia Naturalis
Veldman, S..
Medicinal plants are an important source of primary healthcare for many people in Tanzania. These medicinal plants are harvested from the wild, and increasing commercial trade poses a serious threat to local plant populations. Currently it is unknown which species are traded and in what amounts. Across the southwestern border in Zambia, the traditional dish chikanda has transformed from a niche product to being a mainstream delicacy. One of the main ingredients are wild-harvested orchids, and these have become depleted throughout the country as an effect of the increased trade. It is unclear which orchid species are targeted and might be at risk of overharvesting. The aims of my doctorate are to map harvest and trade of Tanzanian medicinal plants and...
Tipo: Book (monograph) Palavras-chave: DNA barcoding; Africa; Species delimitation; Orchids; Medicinal plants.
Ano: 2018 URL: http://www.repository.naturalis.nl/record/668031
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Morphological difference between upperside and underside leaf-mining larvae of Phyllocnistis Unipunctella (Stephens, 1834) (Lep. Gracillariidae) and its changing phenology Naturalis
Jordan, M.P.; Langmaid, J.R.; Doorenweerd, C..
Larvae of Phyllocnistis unipunctella (Stephens) mining the upperside and underside of leaves of Populus spp. were compared in terms of gross morphology and the COI DNA barcoding section of mitochondrial DNA. It was discovered that larvae feeding on the underside did not show the dark pigmentation of the prothoracic plate as described in the literature. Larvae were found feeding two months earlier than normal and at least one extra generation was observed. More work isrequired to determine the possibility ofspeciation.
Tipo: Article / Letter to the editor Palavras-chave: Lepidoptera; Prothoracic plate; DNA barcoding; Phenology.
Ano: 2016 URL: http://www.repository.naturalis.nl/record/610600
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Multi-gene phylogenetic analyses reveal species limits, phylogeographic patterns, and evolutionary histories of key morphological traits in Entoloma (Agaricales, Basidiomycota) Naturalis
Morgado, L.N.; Noordeloos, M.E.; Lamoureux, Y.; Gem, J..
Species from Entoloma subg. Entoloma are commonly recorded from both the Northern and Southern Hemispheres and, according to literature, most of them have at least Nearctic-Palearctic distributions. However, these records are based on morphological analysis, and studies relating morphology, molecular data and geographical distribution have not been reported. In this study, we used phylogenetic species recognition criteria through gene genealogical concordance (based on nuclear ITS, LSU, rpb2 and mitochondrial SSU) to answer specific questions considering species limits in Entoloma subg. Entoloma and their geographic distribution in Europe, North America and Australasia. The studied morphotaxa belong to sect. Entoloma, namely species like the notorious...
Tipo: Article / Letter to the editor Palavras-chave: Biodiversity; DNA barcoding; Entolomataceae; Fungal biogeography; Molecular systematics; Phylogenetic species.
Ano: 2013 URL: http://www.repository.naturalis.nl/record/532443
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One fungus, which genes? Development and assessment of universal primers for potential secondary fungal DNA barcodes Naturalis
Stielow, J.B.; Lévesque, C.A.; Seifert, K.A.; Meyer, W.; Irinyi, L.; Smits, D.; Renfurm, R.; Verkley, G.J.M.; Groenewald, M.; Chaduli, D.; Lomascolo, A.; Welti, S.; Lesage-Meessen, L.; Favel, A.; Al-Hatmi, A.M.S.; Damm, U.; Yilmaz, N.; Houbraken, J.; Lombard, L.; Quaedvlieg, W.; Binder, M.; Vaas, L.A.I.; Vu, D.; Yurkov, A.; Begerow, D.; Roehl, O.; Guerreiro, M.; Fonseca, A.; Samerpitak, K.; Diepeningen, A.D. van; Dolatabadi, S.; Moreno, L.F.; Casaregola, S.; Mallet, S.; Jacques, N.; Roscini, L.; Egidi, E.; Bizet, C.; Garcia-Hermoso, D.; Martin, M.P.; Deng, S.; Groenewald, J.Z.; Boekhout, T.; Beer, Z.W. de; Barnes, I.; Duong, T.A.; Wingfield, M.J.; Hoog, G.S. de; Crous, P.W.; Lewis, C.T.; Hambleton, S.; Moussa, T.A.A.; Al-Zahrani, H.S.; Almaghrabi, O.A.; Louis-Seize, G.; Assabgui, R.; McCormick, W.; Omer, G.; Dukik, K.; Cardinali, G.; Eberhardt, U.; Vries, M. de; Robert, V..
The aim of this study was to assess potential candidate gene regions and corresponding universal primer pairs as secondary DNA barcodes for the fungal kingdom, additional to ITS rDNA as primary barcode. Amplification efficiencies of 14 (partially) universal primer pairs targeting eight genetic markers were tested across > 1 500 species (1 931 strains or specimens) and the outcomes of almost twenty thousand (19 577) polymerase chain reactions were evaluated. We tested several well-known primer pairs that amplify: i) sections of the nuclear ribosomal RNA gene large subunit (D1–D2 domains of 26/28S); ii) the complete internal transcribed spacer region (ITS1/2); iii) partial β-tubulin II (TUB2); iv) γ-actin (ACT); v) translation elongation factor 1-α (TEF1α);...
Tipo: Article / Letter to the editor Palavras-chave: DNA barcoding; ITS supplement; Molecular taxonomy; Phylogeny; Species identification; Universal primers.
Ano: 2015 URL: http://www.repository.naturalis.nl/record/588731
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Pharmacognostical study of Desmodium caudatum Anais da ABC (AABC)
LI,JUNNI; LIN,XIAOJING; TANG,GENGQIU; LI,RONG; WANG,DONG; JI,SHENGGUO.
Abstract: Desmodium caudatum (Thunb.) DC, is an ever-green plant widely used in the central and southern China with great economic value for their medical values on fever, dysentery, gastroenteritis, rectal prolapse, snake bites, mastitis, and boils carbuncle. Despite its extensive uses as a traditional Chinese medicine, no systematic research on the identification of Desmodium caudatum has been reported. In this study, traditional pharmacognostical identification including the botanical origin and morphological characters, medicinal material characters, microscopic characters, physicochemical parameters determination and phytochemical screening, and DNA barcoding analysis were employed to establish an accurate and effective identification...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Desmodium caudatum; DNA barcoding; Quality standardization; Traditional pharmacognostical identification.
Ano: 2019 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37652019000300709
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Phyllocnistis hemera sp. nov. (Lepidoptera: Gracillariidae): a new species of leaf-miner associated with Daphnopsis fasciculata (Thymelaeaceae) in the Atlantic Forest Rev. Bras. entomol.
Fochezato,Júlia; Brito,Rosângela; Isaias,Rosy Mary dos Santos; Gonçalves,Gislene Lopes; Moreira,Gilson R.P..
ABSTRACT During recent studies performed in the Atlantic Forest, a new species of Phyllocnistinae (Gracillariidae), Phyllocnistis hemera sp. nov., leaf miner of Daphnopsis fasciculata (Thymelaeaceae) was discovered. The adults are described and illustrated as well as the immature stages, with notes on natural history including a description of the leaf mine. Additionally, DNA barcode sequences were compared to other representatives of Phyllocnistinae to test for the specific status of P. hemera and to infer phylogenetic relationships. This is the fifth species described for the genus Phyllocnistis in the Atlantic Forest and the first record of a gracillarid mining Thymelaeaceae leaves.
Tipo: Info:eu-repo/semantics/article Palavras-chave: Microlepidoptera; New species; Morphology; DNA barcoding; Neotropical region.
Ano: 2018 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0085-56262018000100057
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